Maltose Colorimetric/Fluorometric Assay Kit

Maltose (C₁₂H₂₂O₁₁; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase. The generated glucose can be specifically oxidized to produce a product that interacts with the probe to generate color and fluorescence. Thus, maltose can be determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The kit provides a fast, easy and sensitive method for quantifying maltose in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.).

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian

• Simple procedure; takes ~1 hour • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Maltose

Maltose

none

• Maltose Assay Buffer • Maltose Probe (in DMSO, anhydrous) • α-D-Glucosidase (Lyophilized) • Enzyme Mix (Lyophilized) • Maltose Standard (100 nmol/µl)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, food, growth medium, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.