Fumarate Colorimetric Assay Kit

Catalog number
K633-100
Name
Fumarate Colorimetric Assay Kit
Size
100 assays
Price
480.00 EUR
Supplier

Details

Kit's description
Fumarate (HO₂CCH=CHCO₂H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. BioVision’s Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: Kit can detect as low as 1 nmol of fumarate per well (20 µM).
Kit's benefits
• Simple procedure; takes ~ 40 minutes • Fast and convenient
Category
Fumarate
Kit's other name
none
Contents
• Fumarate Assay Buffer • Fumarate Enzyme Mix • Fumarate Developer • Fumarate Standard (0.1 M)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.