Malate Colorimetric Assay Kit

L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO₂. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others. BioVision has developed an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (λmax = 450 nm). The assay can detect 1 ~ 35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: The assay can detect 1 ~ 35 nmol of Malate in a 50 µl sample with a detection sensitivity ~20 µM.

• Simple procedure; takes ~ 1 hour • Fast and convenient • This kit contains all necessary reagents for a accurate measurement of Malic Acid

Malate Assay Kit

none

• Malate Assay Buffer • Malate Enzyme Mix • WST Substrate • Malate Standard (10 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, food, fruits, cheese, beer and wine samples

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.