PicoProbe™ Lactate Fluorometric Assay Kit

Lactate (CH3CH(OH)COO-) plays an important role in many biological processes. Abnormally high concentrations of lactate have been related to diseases such as diabetes, lactic acidosis, etc. L(+)-Lactate is present in blood and is the major lactate stereoisomer formed in human intermediary metabolism. D-Lactate is also present but only at about 1-5% of L(+)-Lactate concentration. Lactate is a significant energy source for living organisms and can be used to generate cellular ATP. BioVision’s PicoProbe™ Lactate Assay kit is suitable for measuring very low levels of L(+)-lactate in a variety of samples. In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate. This simple, rapid & high-throughput suitable assay kit is the most sensitive Lactate Assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.

• Detection method: Fluorescence (Ex/Em= 535/587 nm) • Applications: The assay can detect L(+):lactate less than 0.2 µM in a variety of biological samples. Analysis of metabolism and cell signaling. Mechanistic study for diabetes.

• Simple, rapid, and high-throughput adaptable. • Kit is the most sensitive Lactate Assay kit on the market.

Lactate Assay

none

• Lactate Assay Buffer • PicoProbe™ (in DMSO) • Lactate Enzyme Mix (lyophilized) • Lactate Substrate Mix (lyophilized) • L(+)-Lactate Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

Inquire

• Serum, plasma, etc. • Animal tissues: Liver, muscle, heart, etc. • Cell culture: Adherent or suspension cells.

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit