Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit

Glycerol-3-Phosphate Dehydrogenase (EC 1.1.1.8) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways. In BioVision’s Glycerol-3-Phosphate Dehydrogenase Activity Assay kit, Glycerol-3-phosphate dehydrogenase forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.

• Detection method: Absorbance (450 nm) • Applications: :Measurement of Glycerol:3:Phosphate Dehydrogenase activity in various tissues/cells :Analysis of fatty acid metabolism and cell signaling :Screening anti:obesity drugs

• Simple, sensitive, and rapid. • The assay can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.

Metabolism

GPDH Activity Colorimetric Assay Kit

• GPDH Assy Buffer • GPDH Substrate (Lyophilized) • GPDH Probe (Lyophilized) • NADH Standard (Lyophilized) • GPDH Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

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• Animal tissues: Liver, brain, heart, kidney, etc. • Cell culture: Preadipocytes, adipocytes, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.