L-Carnitine Colorimetric/Fluorometric Assay Kit

Catalog number
K642-100
Name
L-Carnitine Colorimetric/Fluorometric Assay Kit
Size
100 assays
Price
550.00 EUR
Supplier

Details

Kit's description
Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where β-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active. BioVision's L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the Oxi-Red probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 µM. The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.
Highlights
• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.
Kit's benefits
• Simple procedure; takes ~ 30 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of L-Carnitine levels
Category
Metabolism
Kit's other name
none
Contents
• Carnitine Assay Buffer • Carnitine Probe (in DMSO) • Carnitine Converting Enzyme • Carnitine Substrate Mix • Carnitine Development Mix • Carnitine Standard (10 µmol)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.