Glycogen Colorimetric/Fluorometric Assay Kit

Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in α-1,4 linkage, with branching via α-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. The BioVision Kit is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The assay can detect glycogen 0.0004 to 2 mg/ml.

• Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Glycogen

Glycogen

none

• Hydrolysis Buffer • Development Buffer • OxiRed Probe • Hydrolysis Enzyme Mix • Development Enzyme Mix • Glycogen Standard (2.0 mg/ml)

-20°C

gel pack

12 months

see datasheet

Inquire

• Animal tissues: Liver, muscle etc. • Cell culture: Adherent or suspension cells.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.