Succinate (Succinic Acid) Colorimetric Assay Kit
Catalog number
K649-100
Name
Succinate (Succinic Acid) Colorimetric Assay Kit
Size
100 assays
Price
763.00 EUR
Supplier
Details
Kit's description
Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle. BioVision’s Succinate (Succinic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions & reduces a colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect less than 40 µM of succinate or succinic acid in a variety of sample types.
Highlights
• Detection method: Absorbance (450 nm) • Applications: Measurement of succinate or succinic acid in various samples : Analysis of citric acid cycle in various tissues/cells
Kit's benefits
• Simple, rapid & convenient • The assay can detect less than 40 µM of Succinate or Succinic Acid in various sample types.
Kit's other name
none
Contents
• Succinate Assay Buffer • Succinate Converter (Lyophilized) • Succinate Enzyme Mix (Lyophilized) • Succinate Substrate Mix (Lyophilized) • Succinate Developer (Lyophilized) • Succinate Standard (Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of succinate or succinic acid in various samples • Analysis of citric acid cycle in various tissues/cells
Samples tested
• Animal tissues such as liver, muscle, heart, spleen, lung etc. • Cell culture: adherent or suspension cells • Wine
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.