Acetoacetate Colorimetric Assay Kit

Acetoacetate (AcAc), a β-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AcAc can be enzymatically reduced to 3-β-hydroxybutyrate (β-HB), or decarboxylated producing acetone (CH3)2CO). Ketone bodies (βHB: 78%; AcAc: 20% & CH3)2CO: 2%) are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Traditionally, AcAc levels have been qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore. BioVision’s Acetoacetate Assay Kit has adapted that principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine. In this non-enzymatic assay, AcAc reacts with a substrate to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-β-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 µM.

• Detection method: Absorbance (550 nm) • Applications: : Measurement of acetoacetate in various biological samples • Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.

• Specific for acetoacetate and does not detect 3-β-hydroxybutyrate • The assay can detect less than 25 µM of acetoacetate in various sample types.

Acetoacetate

none

• Acetoacetate Assay Buffer • Acetoacetate Standard (Lyophilized) • Acetoacetate Substrate

-20°C

gel pack

12 months

see datasheet

• Measurement of acetoacetate in various biological samples. • Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.

• Serum, plasma, blood, urine & other body fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.