PicoProbe™ β-Hydroxybutyrate Fluorometric Assay Kit

β-Hydroxybutyrate (β-HB) is the most abundant of the ketone bodies (~78% of total ketone bodies in blood). Ketoacidosis (high concentrations of the ketone bodies) can be caused by a variety of conditions, such as diabetes, alcoholism and severe starvation. Ketoacidosis can be fatal if not treated. Additionally, ketogenic diets can increase the concentration of ketone bodies slightly in healthy individuals. BioVision’s β-Hydroxybutyrate Assay Kit offers simplicity, enhanced sensitivity, and can be adapted to high-throughput applications. The assay is based on enzymatic oxidation of β-HB that results in generation of fluorescent signal (Ex/Em = 535/587 nm) which is directly proportional to the amount of β-HB. The kit offers an excellent alternative for measurement of β-HB when biological sample quantities are limited or subketogenic levels are suspected. The assay can detect β-Hydroxybutyrate as low as 4 µM in a variety of biological samples.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Applications: : Measurement of β:Hydroxybutyrate in various biological samples : Analysis of β:Hydroxybutyrate in pathological conditions

• Simple, rapid & high-throughput adaptable • Highly sensitive: The assay can detect less than 4 µM of β-Hydroxybutyrate (β-HB) in various sample types.

Beta-hydroxybutyrate

PicoProbe™ 3-Hydroxybutyrate Fluorometric Assay Kit

• β-HB Assay Buffer • PicoProbe™ (in DMSO) • β-HB Enzyme Mix (Lyophilized) • β-HB Substrate Mix (Lyophilized) • β-HB Standard (Lyophilized)

-20°C

gel pack

12 months

see datasheet

• Measurement of β-Hydroxybutyrate in various biological samples • Analysis of β-Hydroxybutyrate in pathological conditions

• Serum, plasma • Urine or other body fluids • Food such as milk etc.

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit