Formate Colorimetric Assay Kit

Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate. In the Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Applications: The assay can detect formate in the range from 0.5 to 10 nmols/well.

• Simple procedure; takes ~60 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of formate levels in a number of sample types

Formate, Formic acid

none

• Formate Assay Buffer • Formate Enzyme Mix • Formate Substrate Mix • Formate Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture lysates, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.