Isocitrate Colorimetric Assay Kit

Isocitric acid (HOOC-CHOH-CH (-COOH)-CH₂-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. BioVision's Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. The Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 – 5 µg) of isocitrate.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 – 5 µg) of isocitrate.

• Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of isocitrate levels

Isocitrate

none

• Isocitrate Assay Buffer • Isocitrate Enzyme Mix • Substrate Mix • Isocitrate Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.