Succinate Dehydrogenase Activity Colorimetric Assay Kit

Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications. BioVision’s Succinate Dehydrogenase Activity Assay kit is rapid, simple and high-throughput adaptable. In this assay, Succinate dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity). This assay kit can detect less than 0.1mU Succinate Dehydrogenase Activity in a variety of samples.

• Detection method: Absorbance (OD = 600 nm) • Applications: • Measurement of Succinate Dehydrogenase Activity in various tissues/cells. • Analysis of citric acid cycle

• Simple & rapid • High-throughput adaptable • This assay kit can detect less than 0.1mU Succinate Dehydrogenase Activity in a variety of samples.

Succinate Dehydrogenase

Succinate-Coenzyme Q Reductase Activity Colorimetric Assay Kit, Complex II Activity Colorimetric Assay Kit

• SDH Assay Buffer • SDH Substrate Mix (Lyophilized) • SDH Probe • DCIP Standard (2 mM) • SDH Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

• Measurement of Succinate Dehydrogenase Activity in various tissues/cells. • Analysis of citric acid cycle

• Animal tissues: heart, liver, muscle, etc. • Purified mitochondria • Cell culture: Adherent or suspension cells

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.