PicoProbe™ Lactulose Fluorometric Assay Kit

Lactulose is a synthetic disaccharide composed of galactose and fructose. It is closely related to lactose which is a disaccharide consisting of galactose and glucose. Since Lactulose is not absorbed by the gut; it is therefore used as a laxative and for hepatic encephalopathy and is considered a prebiotic. It is detectable in heated milk since it is easily formed by isomerization of lactose at elevated temperatures or in alkaline solutions. Significant background is observed in traditional assays where glucose formed from the fructose is used to measure the lactulose concentration in the samples. BioVision’s Lactulose Assay Kit provides a simple, sensitive, & convenient assay to measure Lactulose. In this kit, fructose is directly measured using Lactulose Enzyme Mix after hydrolysis of Lactulose into galactose and fructose. The linear range of the assay is between approximately 10 pmol and 1 nmol.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Applications: Determination of lactulose

• Simple, sensitive & convenient. • The linear range of the assay is between approximately 10 pmol and 1 nmol.

Lactulose

none

• Lactulose Hydrolysis Buffer • Hydrolysis Enzyme Mix (Lyophilized) • Lactulose Reaction Buffer • Lactulose Enzyme Mix (Lyophilized) • PicoProbe™ • Enhancement Solution • Lactulose Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

Determination of lactulose

• Pharmaceuticals, foods, and dairy products

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit