Glucose Uptake Fluorometric Assay Kit

Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to BioVision’s PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Species reactivity: Mammalian • Applications: This easy to use non:radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.

• Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive

Glucose Assay

none

• Extraction Buffer • Neutralization Buffer • 2-Deoxyglucose (2-DG, 10 mM) • 2-DG Uptake Assay Buffer • Enzyme Mix (Lyophilized) • PicoProbe™ (in DMSO) • 2-DG6P Standard (Lyophilized)

-20°C

gel pack

12 months

see datasheet

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Cell lysates

Biovision supplies other types of Assays as 1.