D-Lactate Colorimetric Assay Kit

Catalog number
K667-100
Name
D-Lactate Colorimetric Assay Kit
Size
100 assays
Price
480.00 EUR
Supplier

Details

Kit's description
D-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy. BioVision’s D-Lactate Assay Kit provides a fast, easy way to accurately measure D-lactate in a variety of biological samples. In the D-Lactate Assay Kit, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media. The useful concentration range in samples is 0.01 mM – 10 mM D-lactate.
Highlights
• Detection method: Absorbance (450 nm) • Sample type: • Species reactivity: Mammalian • Application: The useful concentration range in samples is 0.01 mM – 10 mM D:lactate.
Kit's benefits
• Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Lactate
Category
Lactate Assay
Kit's other name
none
Contents
• D-Lactate Assay Buffer • D-Lactate Enzyme Mix • D-Lactate Substrate Mix • D-Lactate Standard (100 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.