PicoProbe™ D-Lactate Fluorometric Assay Kit

D-Lactate is the result of anaerobic glycolysis by microorganisms in the gastrointestinal system, and the product of detoxification of methylglyoxal by the glyoxalase system. The presence of abnormal levels of D-Lactate has been linked to a series of pathological conditions, such as diabetes, and appendicitis. BioVision’s PicoProbeTM D-Lactate Assay kit offers simplicity, sensitivity, and can be adapted to high-throughput research. The assay enzymatically oxidizes D-Lactate generating a fluorescent signal (Ex/Em = 535/587 nm). The signal is directly proportional to the amount of D-Lactate. The assay kit can detect samples containing as low as 0.1 µM D-Lactate.

• Detection method: Fluorescence (Ex/Em= 535/587 nm) • Applications: :Measurement of D:Lactate in various tissues/cells/biological fluids :Analysis of D:Lactate in pathological conditions :Mechanistic study of the glyoxalase system

• Simple, highly sensitive, and high-throughput adaptable. • The assay kit can detect samples containing as low as 0.1 µM D-Lactate.

Lactate Assay

none

• D-Lactate Assy Buffer • PicoProbe™ (in DMSO) • D-Lactate Enzyme Mix (Lyophilized) • D-Lactate Substrate Mix (Lyophilized) • D-Lactate Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

Inquire

• Serum, plasma, urine & other body fluids • Animal tissues: liver, muscle, heart, etc. • Cell culture: adherent or suspension cells • Fermentation media • Food

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit