Ascorbic Acid Colorimetric Assay Kit II (FRASC)

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference. BioVision’s FRASC Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Feᶟᶧ is reduced to Fe²ᶧ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.

• Detection method: Absorbance (545:600 nm) • Species reactivity: Mammalian • Application: The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.

• Simple procedure; takes ~ 30 minutes to complete the assay • Fast and convenient

Ascorbic Acid Assay

none

• FRASC Buffer • Ascorbic Acid Probe • FeCl3 solution • Ascorbate Oxidase (lyophilized) • Ascorbic Acid Standard (20 µmole)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.