Heme Colorimetric Assay Kit

Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (< 1µM ≈ 650 ng/ml) exerting regulatory functions such as repression of nonspecific δ-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces. BioVision’s Hemin Assay Kit utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (λ = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.

• Detection method: Absorbance (570 nm) • Species reactivity: Mammalian • Application: Trace amounts of Hemin can be quantitated in the 5:160 pg (10:250 fmol) range.

• Simple procedure; takes ~ 30 minutes to complete the assay • Fast and convenient • Highly sensitive assays for measuring Heme level in biological samples

Heme

none

• Hemin Assay Buffer • Hemin Probe • DMSO (anhydrous) • Enzyme Mix • Hemin Substrate • Hemin Standard (1 nmol)

4°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.