PicoProbeTM Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit

Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes. Biovision’s PicoProbeTM Dihydroxyacetone Phosphate Assay kit is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, TPI converts DHAP to GAP that undergoes a series of reaction and reduces PicoProbe™ to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of Dihydroxyacetone Phosphate. This simple, sensitive, and easy to use assay kit can detect Dihydroxyacetone Phosphate as low as 0.5 µM in a variety of samples.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Application: Measurement of Dhydroxyacetone Phosphate in various tissues/cells • Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis • Mechanistic study of human TPI deficiency

• Simple, Sensitive & Rapid protocol • Highly-Sensitive: Detects as low as 0.5 µM DHAP

Dihydroxyacetone Phosphate, DHAP, Glycerone Phosphate

PicoProbeTM Glycerone Phosphate Colorimetric Assay Kit

• DHAP Assay Buffer • PicoProbe™ (in DMSO) • DHAP Enzyme Mix • DHAP Developer • DHAP Standard

-20°C

gel pack

12 months

see datasheet

• Measurement of Dhydroxyacetone Phosphate in various tissues/cells • Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis • Mechanistic study of human TPI deficiency

Animal tissues: e.g., liver, muscle etc. • Cell culture: adherent or suspension cells • Biological fluids: serum, plasma etc.

Biovision supplies other types of Assays as 1.