Sulfatase Activity Assay Kit (Colorimetric)

Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer. BioVision’s Sulfatase Activity Assay Kit provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at 515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.

• Detection method: Absorbance (515 nm) • Application : Measurement of sulfatase activity

• Simple, Sensitive & Rapid protocol • Highly-Sensitive: Detects as low as 1 mU sulfatase in various sample types

Other metabolism-related assays, Sulfatase assay

none

• Sulfatase Assay Buffer • Sulfatase Substrate • Stop/Developing Solution • Sulfatase • 4-Nitrocatechol Standard (0.5 mM)

-20°C

gel pack

12 months

see datasheet

Measurement of sulfatase activity

• Animal tissues such as liver, endometrium, and breast cancer • Adherent or suspension cells • Purified enzyme

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.