Sulfatase Activity Assay Kit (Colorimetric)

Catalog number
K675-100
Name
Sulfatase Activity Assay Kit (Colorimetric)
Size
100 assays
Price
611.00 EUR
Supplier

Details

Kit's description
Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer. BioVision’s Sulfatase Activity Assay Kit provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at 515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.
Highlights
• Detection method: Absorbance (515 nm) • Application : Measurement of sulfatase activity
Kit's benefits
• Simple, Sensitive & Rapid protocol • Highly-Sensitive: Detects as low as 1 mU sulfatase in various sample types
Category
Other metabolism-related assays, Sulfatase assay
Kit's other name
none
Contents
• Sulfatase Assay Buffer • Sulfatase Substrate • Stop/Developing Solution • Sulfatase • 4-Nitrocatechol Standard (0.5 mM)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Measurement of sulfatase activity
Samples tested
• Animal tissues such as liver, endometrium, and breast cancer • Adherent or suspension cells • Purified enzyme
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.