Glucose Uptake Colorimetric Assay Kit

Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.

• Detection method: Absorbance (412 nm) • Species reactivity: Mammalian • Application: This easy to use non:radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.

• Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive

Glucose Assay

none

• Extraction Buffer • Neutralization Buffer • 2-Deoxyglucose (2-DG, 10 mM) • Assay Buffer • Enzyme Mix (lyophilized) • Recycling Mix (lyophilized) • 2-DG6P Standard (lyophilized) • Glutathione Reductase (lyophilized) • Substrate-DTNB (lyophilized)

-20°C

gel pack

12 months

see datasheet

Inquire

Living cells (suspension and adherent)

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.