PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. BioVision's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. The PicoProbe™ G6P Assay Kit is much more sensitive than the G6P Colorimetric Assay Kit for low G6P samples.

• Detection method: Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1:500 µM in the original sample, in a high:throughput screening (HTS) format.

• Simple procedure; takes ~ 35-40 minutes to complete the assay • Fast and convenient • Sensitive, efficient and accurate assay

Glucose-6-phosphate Assay

none

• G6P Assay Buffer • PicoProbe™ (in DMSO) • G6P Enzyme Mix • G6P Substrate Mix • G6P Standard (10 µmol)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium etc.

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit