PicoProbe™ Fructose-6-Phosphate Fluorometric Assay Kit

Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator (1). BioVision's PicoProbe ™ Fructose-6-phosphate Assay Kit is a fluorescence-based simple, highly sensitive and rapid means of quantifying F6P in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate which is subsequently oxidized with the generation of a fluorescent product. The PicoProbe™ F6P Assay Kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ~1 µM of F6P.

• Detection method: Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The PicoProbe™ F6P Assay Kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ~1 µM of F6P.

• Simple procedure; takes ~ 35-40 minutes to complete the assay • Fast and convenient • Sensitive, efficient and accurate assay

none

• F6P Assay Buffer • F6P Probe • F6P Enzyme Mix • F6P Converter • F6P Developer • F6PStandard (10 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit