Acidic Mammalian Chitinase Inhibitor Screening Kit (Fluorometric)

Acidic Mammalian Chitinase (AMCase) belongs to the glycosyl hydrolase-18 family (EC 3.2.1.14), which is capable of hydrolyzing the β-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. AMCase is relatively abundant in the gastrointestinal tract and lung. It is highly expressed in the lungs of asthmatic patients and asthma animal models. In addition, the change in AMCase expression can be used to monitor Th-2/IL-13 mediated immune response and inflammation. Inhibition of AMCase activity has been considered as a therapeutic strategy to alleviate symptoms of allergic airway inflammation. Thus, there is a need to identify AMCase inhibitors as potential candidates to treat and/or prevent asthma. Biovision’s Acidic Mammalian Chitinase Inhibitor Screening Kit uses Bisdionin F— a highly selective AMCase inhibitor as positive control and it provides a simple, rapid, sensitive and reliable test suitable for screening/characterizing AMCase inhibitors.

• Detection method: Fluorescence (RFU= 320/445 nm) • Application:Screening/characterizing/studying Mammalian Chitinase inhibitors/activators

• Simple and reliable test to screen AMCase inhibitors/activators • High-throughput compatible • Includes Inhibitor Control (Bisdionin F)

Protease

none

• AMCase Assay Buffer • AMCase Substrate (in DMSO) • Acidic Mammalian Chitinase (lyophilized) • Bidionin F (5 mM)

-20°C

gel pack

12 months

see datasheet

Screening/characterizing/studying Mammalian Chitinase inhibitors/activators

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.