Glucose-1-Phosphate Colorimetric Assay Kit

Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P  G1P), UDPG-pyrophosphorylase (G1P  UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n]  UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1µM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: The kit can detect glucose:1:phosphate concentrations in the range of 1 µM – 10 mM.

• Simple procedure; takes ~ 40 minutes • Rapid, convenient and sensitive • Kit contains all necessary reagents for accurate measurement of glucose-1-phosphate levels

Glucose-1-Phosphate Assay

G1P Colorimetric Assay Kit

• G1P Assay Buffer• G1P Enzyme Mix (Lyophilized)• G1P Developer (Lyophilized)• G1P Substrate Mix (Lyophilized)• G1P Standard (Lyophilized)

-20°C

gel pack

12 months

see datasheet

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• Animal tissues: Liver, muscle and heart etc. • Cell culture: Adherent or suspension cells.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.