Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric)

NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP, EC:1.1.1.80) belongs to the superfamily of alcohol dehydrogenases (Alcohol DHs, ADHs, EC 1.1.1.1) with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Isopropanol dehydrogenase facilitates the conversion between secondary alcohols to aldehydes and ketones with the reduction of NADP+ to NADPH. BioVision’s Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1-NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at λ = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.

• Detection method: Absorbance (450 nm) • Sample Type: Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc., Animal tissues: such as liver, pancreas, small intestine, etc. Animal cells. • Applications: Measurement of Isopropanol Dehydrogenase (ADH1:NADP) activity in various cells, Measurement of NADP:dependent alcohol dehydrogenases’ activity with broad substrate specificity in various tissues/cells.

Simple, colorimetric protocol. Detects ADH1-NADP activity as low as 0.001 mU in samples. Compatible with multiple sample types.

Alcohol Dehydrogenase

Alcohol Dehydrogenase (NADP dependant) Activity Assay Kit (Colorimetric)

ADH1-NADP Assay Buffer ADH1-NADP Substrate ADH1-NADP Developer ADH1-NADP Positive Control NADPH Standard

-20°C

gel pack

12 months

Mammalian samples. Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc.

• Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells. • Measurement of NADP-dependent alcohol dehydrogenases’ activity with broad substrate specificity in various tissues/cells.

• Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc. • Animal tissues: such as liver, pancreas, small intestine, etc. • Animal cells.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.