Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

Catalog number
K709-100
Name
Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit
Size
100 assays
Price
561.00 EUR
Supplier

Details

Kit's description
Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.
Highlights
• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit detects 0.1 mU/ml pyruvate kinase.
Kit's benefits
• Simple procedure; takes ~ less than 40 minutes • Fast and convenient
Category
Pyruvate
Kit's other name
none
Contents
• PK Assay Buffer • OxiRed™ Probe (in DMSO) • PK Enzyme Mix • PK Substrate Mix • PK Positive Control (~18 mU) • Pyruvate Standard (100 nmol/µl)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Purified/Isolated enzyme, Testing inhibitor panels
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.