Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit detects 0.1 mU/ml pyruvate kinase.

• Simple procedure; takes ~ less than 40 minutes • Fast and convenient

Pyruvate

none

• PK Assay Buffer • OxiRed™ Probe (in DMSO) • PK Enzyme Mix • PK Substrate Mix • PK Positive Control (~18 mU) • Pyruvate Standard (100 nmol/µl)

-20°C

gel pack

12 months

see datasheet

Inquire

Purified/Isolated enzyme, Testing inhibitor panels

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.