Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice. BioVision has developed an easy and sensitive assay to determine XO in variety of samples. In the assay, XO oxidizes xanthine to hydrogen peroxide (H₂O₂) which reacts stoichiometrically with OxiRed™ Probe to generate color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.

• Detection method:Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit detects 1:100 mU xanthine oxidase in 100 µl reaction volume.

• Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Xanthine Oxidase activity in various biological samples

Xanthine

none

• XO Assay Buffer • OxiRed™ Probe • DMSO (anhydrous) • XO Enzyme Mix • XO Substrate Mix • XO Positive Control (~200mU) • H₂O₂ Standard (0.88 M)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.