Aconitase Activity Colorimetric Assay Kit

Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe₄S₄]²ᶧ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian

• Simple procedure; takes ~ less than 40 minutes • Fast and convenient • Sensitive assays for measuring Aconitase activity in various biological samples.

Aconitase

none

• Assay Buffer • Substrate • Developer • Enzyme Mix (200µl) • Cysteine • (NH₄)₂Fe(SO₄)₂ • Isocitrate Standard (10µmol)

4°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.