Lipase Activity Colorimetric Assay Kit II

Lipase is a subclass of the esterases that catalyze the hydrolysis of ester bonds in water-insoluble, lipid substrates. Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. triglycerides, fats, oils) in most, if not all, living organisms. In humans, pancreatic lipases are the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. During the damage of the pancreas, lipase levels can rise 5 to 10-fold within 24 to 48 hours. The kit provides a simple, sensitive, and reliable assay for rapid analysis of Lipase in samples. In the assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (λ=412 nm). The kit is also suitable for high throughput analyses.

• Detection method: Absorbance (412 nm) • Species reactivity: Mammalian

• Simple procedure; takes ~ 60-90 minutes • Fast and convenient • Sensitive assays for measuring Lipase activity in various biological samples and the kit is also suitable for high throughput analyses.

Lipase

none

• Lipase Assay Buffer • DTNB Probe • Lipase Substrate • TNB Standard (5 mM) • Lipase Positive Control

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media, food samples, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.