Phospholipase D (PLD) Activity Colorimetric Assay Kit

Phospholipase D (PLD) is a key player in phospholipid metabolism. PLD hydrolyzes the phosphodiester bond of the glycerophospholipids, resulting in the production of phosphatidic acid and a free headgroup. PLD activity regulates the actin cytoskeleton, vesicle trafficking for secretion and endocytosis, and receptor signaling. Abnormalities in PLD activity and expression have been associated with Alzheimer's disease, stroke, cancer and other brain disorders. BioVision’s Phospholipase D Activity Assay Kit provides a simple and sensitive method of measuring PLD activity using colorimetry (OD 570 nm). In this assay, PLD cleaves choline from Phosphatidylcholine. The free choline is then oxidized by PLD enzyme mix to generate an intermediate which reacts with PLD Probe to generate color (OD 570 nm). This high-throughput adaptable assay kit can detect PLD activity as low as 1.0 mU/ml in a variety of samples.

• Detection method: Absorbance (OD 570 nm) • Applications: : Measurement of PLD activity in various tissues/cells : Analyzing intracellular signaling : Screening of PLD inhibitors

• Simple, rapid & convenient • The assay can detect phospholipase D (PLD) Activity less than 1 mU/ml in various sample types.

Phospholipase D

Lecithinase D Activity Colorimetric Assay Kit, Choline Phosphatase Activity Colorimetric Assay Kit, Lipophosphodiesterase II Activity Colorimetric Assay Kit

• PLD Assay Buffer • PLD Probe (in DMSO) • PLD Substrate • PLD Enzyme Mix (Lyophilized) • Choline Standard (Lyophilized) • PLD Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

• Measurement of PLD activity in various tissues/cells • Analyzing intracellular signaling • Screening of PLD inhibitors

• Animal tissues: liver, brain, kidney, etc. • Cell culture: Adherent or suspension cells

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.