Glutamate Dehydrogenase Activity Colorimetric Assay Kit

Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes. BioVision’s GDH Assay Kit provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.

• Simple procedure • Fast and convenient • Sensitive assays for measuring GDH activity in various biological samples and the kit is also suitable for high throughput analyses.

GDH

none

• GDH Assay Buffer • Glutamate (2 M) • GDH Developer • GDH Positive Control • NADH (0.5 μmol)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.