PicoProbe™ Lactate Dehydrogenase Activity Assay Kit

Lactate Dehydrogenase (LDH: EC 1.1.1.27) is a common enzyme found in wide range of organisms. It catalyzes the interconversion of pyruvate and lactate, with concomitant interconversion of NADH and NAD+. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries. BioVision’s PicoProbe™ Lactate Dehydrogenase Activity Assay kit provides a quick and easy method for monitoring Lactate Dehydrogenase activity in a wide variety of samples. In this assay, Lactate Dehydrogenase converts lactate into pyruvate and NADH, which reacts with PicoProbe™ to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, highly sensitive and high-throughput adaptable, and can detect Lactate Dehydrogenase activity as low as 1 µU/ml.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Application: • Measurement of LDH activity in various tissues and cells • Evaluation of tissue damages or cell cytotoxicity

• Simple & highly sensitive • High-throughput adaptable • Detection Limit: 1 µU/ml

Lactate Dehydrogenase, LDH

none

• LDH Assay Buffer • LDH Substrate Mix (Lyophilized) • PicoProbe™ (in DMSO) • NADH Standard (Lyophilized) • LDH Positive Control

-20°C

gel pack

12 months

see datasheet

• Measurement of LDH activity in various tissues and cells • Evaluation of tissue damages or cell cytotoxicity

• Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: adherent or suspension Cells • Culture medium & fermentation • Human serum or plasma

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit