Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis. Therefore the early detection of ALDH activity levels can be prognostic and guide the therapeutic strategies. The BioVision Aldehyde Dehydrogenase (ALDH) Activity Assay Kit is a simple, fast and reliable method to quantify the ALDH enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay can detect < 0.5 mU of ALDH activity (based on our unit definition) in a variety of samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: The assay can detect < 0.5 mU of ALDH activity (based on our unit definition) in a variety of samples.

• Simple procedure • Fast and convenient • Sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.

ALDH

none

• ALDH Assay Buffer • Acetaldehyde • ALDH Substrate Mix (Lyophilized) • ALDH Positive Control (Lyophilized) • NADH Standard (0.5 µmol, Lyophilized)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, cell lysates, plasma and serum, as well as many other biological fluids etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.