Neuraminidase Activity Fluorometric Assay Kit

Neuraminidase (NA) is a very common enzyme that hydrolyzes terminal sialic acid residues on polysaccharide chains; most often a galactose residue. NA activity plays a key role in the invasion of target cells and the replication of influenza virus. NA activity also assists in the elution of progeny viruses from infected cells, and prevents self-aggregation of virus. Thus, NA is an important target for drug development. BioVision’s Neuraminidase Activity Assay Kit provides a simple and sensitive method for measuring NA activity using fluorescence (Ex/Em = 530/590 nm). The assay utilizes NA Probe to detect the neuraminidase activity. This high-throughput adaptable assay kit can detect NA activity as low as 2.0 mU/ml in a variety of samples.

• Detection method: fluorescence (Ex/Em = 530/590 nm) • Application: Measurement of NA activity in various tissues/cells extracts or serum sample infected with influenza virus.

• Simple, rapid & convenient • The assay can detect Neuraminidase activity < 2 mU/ml in a variety of samples.

Neuraminidase

none

• NA Assay Buffer • NA Probe (in DMSO, anhydrous) • NA Substrate (Lyophilized) • NA Enzyme Mix I (Lyophilized) • NA Enzyme Mix II (Lyophilized) • Galactose Standard (100 nmol/µl) • NA Positive Control

-20°C

gel pack

12 months

see datasheet

• Measurement of NA activity in various tissues/cells extracts or serum sample infected with influenza virus.

• Animal tissues: liver, brain, kidney etc. • Cell culture: Adherent or suspension cells • Serum

Biovision supplies other types of Assays as 1.