β-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric)

β-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and diagnosis of AKI. BioVision’s NAG Activity Assay Kit provides a simple and sensitive method for monitoring NAG enzymatic activity. In this assay, NAG uses a synthetic p-nitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance (OD 400 nm). The assay can detect as low as 50 µU of NAG activity in a variety of samples.

• Detection method: Absorbance (400 nm) • Application: Measurement of NAG in biological samples from different mammalian species

• Convenient & sensitive • The assay can detect as low as 50 µU of NAG activity in a variety of samples

Metabolism, NAG activity

NAG Activity Assay Kit (Colorimetric)

• NAG Assay Buffer • NAG Substrate • p-Nitrophenol (pNP) (20 mM) • NAG Stop Solution • NAG Positive Control

-20°C

gel pack

12 months

see datasheet

• Measurement of NAG in biological samples from different mammalian species

• Biological fluids such as urine, serum etc. • Tissues such as kidney, liver, brain etc. • Cells such as HeLa, HepG2, CHO etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.