Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. BioVision’s Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

• Detection method:Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm) • Species reactivity: All • Applications: The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.

• Simple procedure • Fast and convenient • Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples

Peroxidase

none

• MDA Lysis Buffer • Phosphotungstic Acid Solution • BHT (100X) • TBA • MDA Standard (4.17 M)

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, plasma and other biological fluids (optimized by end user)

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.