PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)

Catalog number
K751-100
Name
PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)
Size
100 assays
Price
591.00 EUR
Supplier

Details

Kit's description
Glucose-6-Phosphate Dehydrogenase (G6PDH: EC 1.1.1.49) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as liver, mammary gland, adipose tissue, and adrenal gland. BioVision’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces PicoProbe™ to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 µU of G6PDH activity.
Highlights
• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Application: Measurement of G6PDH activity in various tissues and cells : Evaluation of pentose phosphate pathway
Kit's benefits
• Rapid & simple • High-throughput adaptable • Can detect as low as 1 µU of G6PDH activity
Kit's other name
PicoProbe™ G6PDH Activity Assay Kit (Fluorometric)
Contents
• G6PDH Assay Buffer • PicoProbe™ (in DMSO) • G6PDH Substrate • G6PDH Developer • G6PDH Positive Control • NADPH Standard (200 nmol)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of G6PDH activity in various tissues and cells • Evaluation of pentose phosphate pathway
Samples tested
• Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: adherent or suspension cells • Plant tissues
Test
Biovision supplies other types of Assays as 1.
Conjugate
Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit