PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)

Glucose-6-Phosphate Dehydrogenase (G6PDH: EC 1.1.1.49) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as liver, mammary gland, adipose tissue, and adrenal gland. BioVision’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces PicoProbe™ to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 µU of G6PDH activity.

• Detection method: Fluorescence (Ex/Em = 535/587 nm) • Application: Measurement of G6PDH activity in various tissues and cells : Evaluation of pentose phosphate pathway

• Rapid & simple • High-throughput adaptable • Can detect as low as 1 µU of G6PDH activity

PicoProbe™ G6PDH Activity Assay Kit (Fluorometric)

• G6PDH Assay Buffer • PicoProbe™ (in DMSO) • G6PDH Substrate • G6PDH Developer • G6PDH Positive Control • NADPH Standard (200 nmol)

-20°C

gel pack

12 months

see datasheet

• Measurement of G6PDH activity in various tissues and cells • Evaluation of pentose phosphate pathway

• Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: adherent or suspension cells • Plant tissues

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit