Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit

Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: α-ketoglutarate + alanine ⇌ glutamate + pyruvate It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity: Mammalian • Applications: The kit detects ALT with a detection limit of 10 mU per well.

• Simple procedure • Fast and convenient • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.

ALT

none

• ALT Assay Buffer • OxiRed Probe (in DMSO) • ALT Enzyme Mix • ALT Substrate • Pyruvate Standard (100 nmol/µl) • ALT positive control

-20°C

gel pack

12 months

see datasheet

• Evaluation of pentose phosphate pathway

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.