Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit

Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s AST Assay Kit, an amino group is transferred of from aspartate to ⍺-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of AST with a detection limit of 10 mU per well.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: This assay detects AST/ SGOT with a detection limit of 10 mU per well.

• Simple procedure • Fast, reliable and convenient • Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses.

AST, SGOT

none

• AST Assay Buffer • AST Enzyme Mix • Developer • AST Substrate • Glutamate Standard (0.1M) • AST Positive Control

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.