Asparaginase Activity Colorimetric/Fluorometric Assay Kit

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme’s antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy. BioVision provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (λ=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.

• Detection method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/590 nm)

• Simple procedure; takes 40 minutes • Fast, reliable and convenient • Kit can be used to detect Asparaginase enzyme’s antineoplastic effects, based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine.

Asparaginase

none

• Asparaginase Assay Buffer • OxiRed™ Probe (in DMSO) • Substrate Mix • Aspartate Enzyme Mix • Conversion Mix • Positive Control • Aspartate Standard (100 mM)

-20°C

gel pack

12 months

see datasheet

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Bacteria, plants and many animals (e.g. guinea pigs) cell lysates, biological samples, cancer cell lysates, food samples, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.