Isocitrate Dehydrogenase Activity Colorimetric Assay Kit

Catalog number
K756-100
Name
Isocitrate Dehydrogenase Activity Colorimetric Assay Kit
Size
100 assays
Price
449.00 EUR
Supplier

Details

Kit's description
It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: This assay has a detection sensitivity as low as 0.01 mU.
Kit's benefits
• Simple procedure • Fast and convenient • Sensitive assays for measuring IDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit's other name
none
Contents
• IDH Assay Buffer • NADᶧ (lyophilized) • NADPᶧ (lyophilized) • IDH Substrate (lyophilized) • Developer (lyophilized) • IDH Positive Control (NADPᶧ) • NADH Standard (0.5 μmol, lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
Inquire
Samples tested
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.