Isocitrate Dehydrogenase Activity Colorimetric Assay Kit

It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: This assay has a detection sensitivity as low as 0.01 mU.

• Simple procedure • Fast and convenient • Sensitive assays for measuring IDH activity in various biological samples and the kit is also suitable for high throughput analyses.

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• IDH Assay Buffer • NADᶧ (lyophilized) • NADPᶧ (lyophilized) • IDH Substrate (lyophilized) • Developer (lyophilized) • IDH Positive Control (NADPᶧ) • NADH Standard (0.5 μmol, lyophilized)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.