Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit

Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. BioVision's glucose-6-phosphate dehydrogenase Assay Kit is a simple, sensitive and rapid assay detects the activity of G6PDH in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450nm. The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.

• Detection method: Absorbance (450 nm) • Application: Measurement of G6PDH activity in various tissues and cells : Evaluation of pentose phosphate pathway

• Simple procedure; takes ~ 30-60 minutes • Fast and convenient

Glucose-6-Phosphate Dehydrogenase

none

• G6PDH Assay Buffer • G6PDH Substrate • G6PDH Developer • G6PDH Positive Control • NADH Standard (0.5 µmol)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.