Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit

Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In Biovision’s Enteropeptidase Activity Assay Kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU.

• Detection method : Fluorescence (Ex/Em = 380/500 nm) • Application: : Measurement of Enteropeptidase activity in biological samples or purified Enteropeptidase activity. : Removing tag from recombinant proteins having recognition motif.

• Simple & convenient procedure. • Can detect enteropeptidase activity as low as 1 mU. • Kit contains all necessary reagents for measuring enteropeptidase activity.

Enteropeptidase/Enterokinase

none

• Enteropeptidase Assay Buffer • Enteropeptidase Substrate (10 mM, in DMSO) • Human Enteropeptidase (Positive Control) • AFC Standard (1 mM)

-20°C

gel pack

12 months

see datasheet

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Biovision supplies other types of Assays as 1.