Glutathione Reductase Activity Colorimetric Assay Kit

Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. BioVision’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples.

• Detection method: Absorbance (405 nm) • Species reactivity: Mammalian • Application: The assay can detect 0.1 – 40 mU/ml GR in various samples.

• Simple procedure; takes ~ 30-40 minutes • Fast and convenient

Glutathione reductase

none

• GR Assay Buffer • 3% H₂O₂ • Catalase • TNB Standard (2.5 µmol) • DTNB • NADPH-GNERAT™ • GSSG • GR Positive Control (10 mU)

-20°C

gel pack

12 months

see datasheet

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Cell and tissue culture supernatants, urine, plasma and serum, erythrocyte lysates, as well as many other biological fluids, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.