Thioredoxin Reductase Activity Colorimetric Assay Kit

Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. BioVision’s Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (λmax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.

• Detection method: Absorbance (412 nm) • Species reactivity: Mammalian

• Simple procedure; takes ~ 30-40 minutes • Fast and convenient

Thioredoxin Reductase

none

• TrxR Assay Buffer • TNB Standard • DTNB • NADPH • TrxR Positive Control • TrxR Inhibitor

-20°C

gel pack

12 months

see datasheet

Inquire

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.