Acetylcholinesterase Activity Colorimetric Assay Kit

Catalog number
K764-100
Name
Acetylcholinesterase Activity Colorimetric Assay Kit
Size
100 assays
Price
510.00 EUR
Supplier

Details

Kit's description
Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. BioVision’s Acetylcholinesterase Activity Assay Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570 nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The intermediate reacts with a highly specific probe to generate color. This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples.
Highlights
• Detection: Absorbance (OD 570 nm) • Application: : Measurement of AChE activity in various tissues/cells : Screening of AChE inhibitors
Kit's benefits
• Rapid, simple & convenient • This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples
Category
Acetylcholinesterase
Kit's other name
AChE Activity Colorimetric Assay Kit, Acetylhydrolase Activity Colorimetric Assay Kit
Contents
• AChE Assay Buffer • AChE Probe (DMSO) • AChE Substrate (Lyophilized) • Choline Oxidase Enzyme Mix (Lyophilized) • Choline Standard (Lyophilized) • AChE Positive Control (Lyophilized)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Applications
• Measurement of AChE activity in various tissues/cells • Screening of AChE inhibitors
Samples tested
• Serum, plasma or blood. • Animal tissues: liver, heart, kidney, etc. • Cell culture: Adherent or suspension cells
Test
Biovision supplies other types of Assays as 1.
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.