Acetylcholinesterase Activity Colorimetric Assay Kit

Acetylcholinesterase (AChE) is one of the most crucial enzymes associated with nerve response and function. AChE is a serine protease that hydrolyzes the neurotransmitter acetylcholine. Irreversible inhibition of AChE may lead to muscular paralysis, convulsions, bronchial constriction, and death by asphyxiation. BioVision’s Acetylcholinesterase Activity Assay Kit provides a simple and sensitive method for continuous monitoring of enzymatic activity using colorimetry (OD 570 nm). In this assay, AChE converts acetylcholine substrate to choline, which is then oxidized by choline oxidase (CO) to produce an intermediate. The intermediate reacts with a highly specific probe to generate color. This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples.

• Detection: Absorbance (OD 570 nm) • Application: : Measurement of AChE activity in various tissues/cells : Screening of AChE inhibitors

• Rapid, simple & convenient • This assay kit can detect acetylcholinesterase activity as low as 0.5 mU/ml in a variety of samples

Acetylcholinesterase

AChE Activity Colorimetric Assay Kit, Acetylhydrolase Activity Colorimetric Assay Kit

• AChE Assay Buffer • AChE Probe (DMSO) • AChE Substrate (Lyophilized) • Choline Oxidase Enzyme Mix (Lyophilized) • Choline Standard (Lyophilized) • AChE Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

• Measurement of AChE activity in various tissues/cells • Screening of AChE inhibitors

• Serum, plasma or blood. • Animal tissues: liver, heart, kidney, etc. • Cell culture: Adherent or suspension cells

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.