PAF Acetylhydrolase Activity Assay Kit (Colorimetric)

Platelet-Activating Factor (PAF or 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is an important phospholipid mediator, which has diverse biological activities. PAF is synthesized and secreted by a variety of cells such as mast cells, monocytes, macrophages etc. Upregulated PAF signaling can cause pathological inflammation and also has been found to be responsible for sepsis, shock, and traumatic injury. PAF Acetylhydrolase (PAF-AH or 1-alkyl-2-acetylglycerophosphocholine esterase or Lipoprotein-associated phospholipase A2 or Lp-PLA2) (EC 3.1.1.47) hydrolyzes PAF by removing acetyl group at the sn-2 position and converts PAF into biologically inactive form, lyso-PAF. PAF-AH has two forms: extracellular and intracellular that shares some similarities. In human, PAF-AH deficiency leads to severe asthma. Therefore early detection of PAF-AH activity is critical for mechanistic study, diagnosis, prevention, and therapeutic purpose. BioVision’s PAF Acetylhydrolase Assay kit provides a quick and easy way for monitoring PAF-AH activity in a variety of samples. In this kit, PAF-AH converts PAF into an inactivated form and probe gets converted into a colored product with strong absorbance at 412 nm. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1mU.

• Detection: Absorbance (OD 412 nm) • Application: : Measurement of PAF:AH activity in various samples : Study/characterize PAF:AH inhibitors or activators : Mechanistic study of inflammatory disorders

• Rapid, simple & convenient • This assay kit can detect PAF Acetylhydrolase activity as low as 0.1 mU in a variety of samples

Lipid Metabolism

Platelet-Activating Factor Acetylhydrolase Activity Assay Kit (Colorimetric)

• PAF-AH Assay Buffer • PAF-AH Substrate • DTNB Probe (in DMSO) • TCEP • PAF-AH Positive Control

-20°C

gel pack

12 months

see datasheet

• Measurement of PAF-AH activity in various samples • Study/characterize PAF-AH inhibitors or activators • Mechanistic study of inflammatory disorders

• Animal tissues such as liver, kidney, etc. • Adherent or suspension cells • Serum or plasma

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.