Phosphoglucomutase Colorimetric Assay Kit

Phosphoglucomutase (PGM) plays a key role in carbohydrate metabolism and widely exists in all organisms. PGM interconverts Glucose-1-Phosphate (G1P) and Glucose-6-Phosphate (G6P) depending on the body requirement. When glycogen breaks down, G1P is generated and phosphoglucomutase converts G1P to G6P, which can go either to glycolytic pathway to generate ATP, or to pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, PGM converts G6P to G1P, which generates glycogen. In humans, phosphoglucomutase have 2 isoforms (PGM I and PGM II). PGM deficiency leads to glucose storage disease. Detection of abnormal phosphoglucomutase activity is crucial for diagnosis, prediction and treatment of the disease. In BioVision’s phosphoglucomutase assay, PGM converts glucose-1-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The Phosphoglucomutase Assay Kit is simple, sensitive and rapid and can detect PGM activity even less than 1 mU/reaction.

• Detection method: Absorbance (450 nm) • Species reactivity: Mammalian • Application: The kit can detect phosphoglucomutase activity even less than 1 mU/reaction.

• Simple procedure • Rapid, Convenient and Reliable • Sensitive assay for measuring phosphoglucomutase activity in various biological samples

Phosphoglucomutase

Glucose phosphomutase Colorimetric Assay Kit, PGM Colorimetric Assay Kit

• PGM Assay Buffer • PGM Substrate (lyophilized) • PGM Enzyme Mix (Lyophilized) • PGM Developer (Lyophilized) • NADH Standard (Lyophilized) • PGM Positive Control (Lyophilized)

-20°C

gel pack

12 months

see datasheet

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• Animal tissues: Muscle, Liver, heart, kidney etc. • Cell culture: Adherent or suspension cells. • Plasma

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.